![]() ![]() The injected samples travel via the GC column, with the differences in chemical properties leading to separation of sample components the separated components of the samples have differences in retention time and elution time because of their different adsorption capacities or because of the differences in partitioning between stationary and mobile phases. Degree of analyte separation can be influenced by the stationary phase properties, column temperature, carrier gas used, and the dimensions of the column (e.g., column diameter, film thickness, column length, etc.). Interactions of stationary phase, mobile phase, and analyte results in components separation. The carrier gas, for example, helium, acts as mobile phase. As the sample, usually in form of vapor, is carried via column of the GC by the heated carrier gas, separation of the components takes place in the column. 8.1, while the GC-MS setup and connection are shown in Fig. ![]()
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